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Toll-like receptor

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Toll-like receptor
The curved leucine-rich repeat region of toll-like receptors, represented here by TLR3
Identifiers
SymbolToll-like receptor
Membranome7
PIRSF037595

Toll-like receptors (TLRs) are a class of proteins that play a key role in the innate immune system. They are single-spanning receptors usually expressed on sentinel cells such as macrophages and dendritic cells, that recognize structurally conserved molecules derived from microbes. Once these microbes have reached physical barriers such as the skin or intestinal tract mucosa, they are recognized by TLRs, which activate immune cell responses. The TLRs include TLR1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, TLR10, TLR11, TLR12, and TLR13. Humans lack genes for TLR11, TLR12 and TLR13[1] and mice lack a functional gene for TLR10.[2] The receptors TLR1, TLR2, TLR4, TLR5, TLR6, and TLR10 are located on the cell membrane, whereas TLR3, TLR7, TLR8, and TLR9 are located in intracellular vesicles (because they are sensors of nucleic acids).[3]

TLRs received their name from their similarity to the protein coded by the toll gene.[4]

Function

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The ability of the immune system to recognize molecules that are broadly shared by pathogens is, in part, due to the presence of immune receptors called toll-like receptors (TLRs) that are expressed on the membranes of leukocytes including dendritic cells, macrophages, natural killer cells, cells of the adaptive immunity T cells, and B cells, and non-immune cells (epithelial and endothelial cells, and fibroblasts).[5]

The binding of ligands — either in the form of adjuvant used in vaccinations or in the form of invasive moieties during times of natural infection — to the TLR marks the key molecular events that ultimately lead to innate immune responses and the development of antigen-specific acquired immunity.[6][7]

Upon activation, TLRs recruit adaptor proteins (proteins that mediate other protein-protein interactions) within the cytosol of the immune cell to propagate the antigen-induced signal transduction pathway. These recruited proteins are then responsible for the subsequent activation of other downstream proteins, including protein kinases (IKKi, IRAK1, IRAK4, and TBK1) that further amplify the signal and ultimately lead to the upregulation or suppression of genes that orchestrate inflammatory responses and other transcriptional events. Some of these events lead to cytokine production, proliferation, and survival, while others lead to greater adaptive immunity.[7] If the ligand is a bacterial factor, the pathogen might be phagocytosed and digested, and its antigens presented to CD4+ T cells. In the case of a viral factor, the infected cell may shut off its protein synthesis and may undergo programmed cell death (apoptosis). Immune cells that have detected a virus may also release anti-viral factors such as interferons.

Toll-like receptors have also been shown to be an important link between innate and adaptive immunity through their presence in dendritic cells.[8] Flagellin, a TLR5 ligand, induces cytokine secretion on interacting with TLR5 on human T cells.[8]

Superfamily

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TIR domain from TLR2. This is a signal transduction domain distinct from the LRR domain discussed earlier.
Main article: Toll-interleukin receptor

TLRs are a type of pattern recognition receptor (PRR) and recognize molecules that are broadly shared by pathogens but distinguishable from host molecules, collectively referred to as pathogen-associated molecular patterns (PAMPs). In addition to the recognition of exogenous PAMPs, TLRs can also bind to endogenous damage-associated molecular patterns (DAMPs) such as heat shock proteins (HSPs) or plasma membrane constituents.[9] TLRs together with the Interleukin-1 receptors form a receptor superfamily, known as the "interleukin-1 receptor / toll-like receptor superfamily"; all members of this family have in common a so-called TIR (toll-IL-1 receptor) domain.

Three subgroups of TIR domains exist. Proteins with subgroup 1 TIR domains are receptors for interleukins that are produced by macrophages, monocytes, and dendritic cells and all have extracellular Immunoglobulin (Ig) domains. Proteins with subgroup 2 TIR domains are classical TLRs, and bind directly or indirectly to molecules of microbial origin. A third subgroup of proteins containing TIR domains consists of adaptor proteins that are exclusively cytosolic and mediate signaling from proteins of subgroups 1 and 2.

Extended family

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TLRs are present in vertebrates as well as invertebrates. Molecular building blocks of the TLRs are represented in bacteria and in plants, and plant pattern recognition receptors are well known to be required for host defence against infection. The TLRs thus appear to be one of the most ancient, conserved components of the immune system.

In recent years TLRs were identified also in the mammalian nervous system. Members of the TLR family were detected on glia, neurons and on neural progenitor cells in which they regulate cell-fate decision.[10]

It has been estimated that most mammalian species have between ten and fifteen types of toll-like receptors. Thirteen TLRs (named simply TLR1 to TLR13) have been identified in humans and mice together, and equivalent forms of many of these have been found in other mammalian species.[11][12][13] However, equivalents of certain TLR found in humans are not present in all mammals. For example, a gene coding for a protein analogous to TLR10 in humans is present in mice, but appears to have been damaged at some point in the past by a retrovirus. On the other hand, mice express TLRs 11, 12, and 13, none of which is represented in humans. Other mammals may express TLRs that are not found in humans. Other non-mammalian species may have TLRs distinct from mammals, as demonstrated by the anti-cell-wall TLR14, which is found in the Takifugu pufferfish.[14] This may complicate the process of using experimental animals as models of human innate immunity.

Vertebrate TLRs are divided by similarity into the families of TLR 1/2/6/10/14/15, TLR 3, TLR 4, TLR 5, TLR 7/8/9, and TLR 11/12/13/16/21/22/23.[14]

TLRs in Drosophila immunity

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The Toll immunity pathway as found in the fruit fly[15][16][17][18]

The involvement of toll signalling in immunity was first demonstrated in the fruit fly, Drosophila melanogaster.[19] Fruit flies have only innate immune responses allowing studies to avoid interference of adaptive immune mechanisms on signal transduction. The fly response to fungal or bacterial infection occurs through two distinct signalling cascades, one of which is the toll pathway and the other is the immune deficiency pathway. The toll pathway is similar to mammalian TLR signalling, but unlike mammalian TLRs, toll is not activated directly by pathogen-associated molecular patterns (PAMPs). Its receptor ectodomain recognizes the cleaved form of the cytokine spätzle, which is secreted in the haemolymph as an inactive dimeric precursor. The toll receptor shares the cytoplasmatic TIR domain with mammalian TLRs, but the ectodomain and intracytoplasmatic tail are different. This difference might reflect a function of these receptors as cytokine receptors rather than PRRs.

The toll pathway is activated by different stimuli, such as gram-positive bacteria, fungi, and virulence factors.[17][20] First, the Spätzle processing enzyme (SPE) is activated in response to infection and cleaves spätzle (spz). Cleaved spätzle then binds to the toll receptor and crosslinks its ectodomains. This triggers conformational changes in the receptor resulting in signalling through toll. From this point forward, the signalling cascade is very similar to mammalian signalling through TLRs. The toll-induced signalling complex (TICS) is composed of MyD88, Tube, and Pelle (the orthologue of mammalian IRAK). Signal from TICS is then transduced to Cactus (homologue of mammalian IκB), phosphorylated Cactus is polyubiquitylated and degraded, allowing nuclear translocation of DIF (dorsal-related immunity factor; a homologue of mammalian NF-κB) and induction of transcription of genes for antimicrobial peptides (AMPs) such as drosomycin.[21]

Drosophila have a total of 9 toll family and 6 spz family genes that interact with each other to differing degrees.[22]

TLR2

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Main article: TLR2

TLR2 has also been designated as CD282 (cluster of differentiation 282).

TLR3

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TLR3 does not use the MyD88 dependent pathway. Its ligand is retroviral double-stranded RNA (dsRNA), which activates the TRIF dependent signalling pathway. To explore the role of this pathway in retroviral reprograming, knock down techniques of TLR3 or TRIF were prepared, and results showed that only the TLR3 pathway is required for full induction of target gene expression by the retrovirus expression vector. This retroviral expression of four transcriptional factors (Oct4, Sox2, Klf4 and c-Myc; OSKM) induces pluripotency in somatic cells. This is supported by study, which shows, that efficiency and amount of human iPSC generation, using retroviral vectors, is reduced by knockdown of the pathway with peptide inhibitors or shRNA knockdown of TLR3 or its adaptor protein TRIF. Taken together, stimulation of TLR3 causes great changes in chromatin remodeling and nuclear reprogramming, and activation of inflammatory pathways is required for these changes, induction of pluripotency genes and generation of human induced pluripotent stem cells (iPSC) colonies.[23]

TLR11

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As noted above, human cells do not express TLR11, but mice cells do. Mouse-specific TLR11 recognizes uropathogenic E.coli and the apicomplexan parasite Toxoplasma gondii. With Toxoplasma its ligand is the protein profilin and the ligand for E. coli is flagellin. The flagellin from the enteropathogen Salmonella is also recognized by TLR11.[24]

As mouse TLR11 is able to recognize Salmonella effectively, normal mice do not get infected by oral Salmonella Typhi, which causes food- and waterborne gastroenteritis and typhoid fever in humans. TLR11 deficient knockout mice, on the other hand, are efficiently infected. As a result, this knockout mouse can act as a disease model of human typhoid fever.[25]

Summary of known mammalian TLRs

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Toll-like receptors bind and become activated by different ligands, which, in turn, are located on different types of organisms or structures. They also have different adapters to respond to activation and are located sometimes at the cell surface and sometimes to internal cell compartments.[26] Furthermore, they are expressed by different types of leucocytes or other cell types:

Receptor Ligand(s)[27] Ligand location[27] Adapter(s) Location Cell types[27]
TLR 1 multiple triacyl lipopeptides Bacterial lipoprotein MyD88/MAL cell surface
TLR 2 multiple glycolipids Bacterial peptidoglycans MyD88/MAL cell surface
multiple lipopeptides and proteolipids Bacterial peptidoglycans
lipoteichoic acid Gram-positive bacteria
HSP70 Host cells
zymosan (Beta-glucan) Fungi
Numerous others
TLR 3 double-stranded RNA, poly I:C viruses TRIF cell compartment
  • Dendritic cells
  • B lymphocytes
TLR 4 lipopolysaccharide Gram-negative bacteria MyD88/MAL/TRIF/TRAM cell surface
several heat shock proteins Bacteria and host cells
fibrinogen host cells
heparan sulfate fragments host cells
hyaluronic acid fragments host cells
nickel[32]
Various opioid drugs
TLR 5 Bacterial flagellin Bacteria MyD88 cell surface
  • monocyte/macrophages
  • a subset of dendritic cells
  • Intestinal epithelium
  • Breast cancer cells
  • B lymphocytes
Profilin[33] Toxoplasma gondii
TLR 6 multiple diacyl lipopeptides Mycoplasma MyD88/MAL cell surface
  • monocytes/macrophages
  • Mast cells
  • B lymphocytes
TLR 7 imidazoquinoline small synthetic compounds MyD88 cell compartment
loxoribine (a guanosine analogue)
bropirimine
resiquimod
single-stranded RNA RNA viruses
TLR 8 small synthetic compounds; single-stranded Viral RNA, phagocytized bacterial RNA(24) MyD88 cell compartment
  • monocytes/macrophages
  • a subset of dendritic cells
  • Mast cells
  • Intestinal epithelial cells (IECs) *only in Crohn's or ulcerative colitis
  • hippocampal interneurons [34]
TLR 9 unmethylated CpG Oligodeoxynucleotide DNA Bacteria, DNA viruses MyD88 cell compartment
  • monocytes/macrophages
  • Plasmacytoid dendritic cells[29]
  • B lymphocytes
TLR 10 triacylated lipopeptides[35] unknown cell surface
TLR 11 Profilin Toxoplasma gondii[39] MyD88 cell compartment[40]
Flagellin Bacteria (E. coli, Salmonella)[24]
TLR 12 Profilin Toxoplasma gondii[41] MyD88 cell compartment
  • Neurons[42]
  • plasmacytoid dendritic cells
  • conventional dendritic cells
  • macrophages
TLR 13[43][44] bacterial ribosomal RNA sequence "CGGAAAGACC" (but not the methylated version)[45] Virus, bacteria MyD88, TAK-1 cell compartment
  • monocytes/macrophages
  • conventional dendritic cells

Ligands

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Toll-Like Receptor (TLR) ligands among RNA and DNA viruses, Gram-positive and Gram-negative bacteria, fungi, and protists

Because of the specificity of toll-like receptors (and other innate immune receptors) they cannot easily be changed in the course of evolution, these receptors recognize molecules that are constantly associated with threats (i.e., pathogen or cell stress) and are highly specific to these threats (i.e., cannot be mistaken for self molecules that are normally expressed under physiological conditions). Pathogen-associated molecules that meet this requirement are thought to be critical to the pathogen's function and difficult to change through mutation; they are said to be evolutionarily conserved. Somewhat conserved features in pathogens include bacterial cell-surface lipopolysaccharides (LPS), lipoproteins, lipopeptides, and lipoarabinomannan; proteins such as flagellin from bacterial flagella; double-stranded RNA of viruses; or the unmethylated CpG islands of bacterial and viral DNA; and also of the CpG islands found in the promoters of eukaryotic DNA; as well as certain other RNA and DNA molecules. As TLR ligands are present in most pathogens, they may also be present in pathogen-derived vaccines (e.g. MMR, influenza, polio vaccines) most commercially available vaccines have been assessed for their inherent TLR ligands' capacity to activate distinct subsets of immune cells.[46][47] For most of the TLRs, ligand recognition specificity has now been established by gene targeting (also known as "gene knockout"): a technique by which individual genes may be selectively deleted in mice.[48][49] See the table above for a summary of known TLR ligands.

Endogenous ligands

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The stereotypic inflammatory response provoked by toll-like receptor activation has prompted speculation that endogenous activators of toll-like receptors might participate in autoimmune diseases. TLRs have been suspected of binding to host molecules including fibrinogen (involved in blood clotting), heat shock proteins (HSPs), HMGB1, extracellular matrix components and self DNA (it is normally degraded by nucleases, but under inflammatory and autoimmune conditions it can form a complex with endogenous proteins, become resistant to these nucleases and gain access to endosomal TLRs as TLR7 or TLR9). These endogenous ligands are usually produced as a result of non-physiological cell death.[50]

Signaling

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Signaling pathway of toll-like receptors. Dashed grey lines represent unknown associations.

TLRs are believed to function as dimers. Though most TLRs appear to function as homodimers, TLR2 forms heterodimers with TLR1 or TLR6, each dimer having a different ligand specificity. TLRs may also depend on other co-receptors for full ligand sensitivity, such as in the case of TLR4's recognition of LPS, which requires MD-2. CD14 and LPS-Binding Protein (LBP) are known to facilitate the presentation of LPS to MD-2.

A set of endosomal TLRs comprising TLR3, TLR7, TLR8 and TLR9 recognize nucleic acid derived from viruses as well as endogenous nucleic acids in context of pathogenic events. Activation of these receptor leads to production of inflammatory cytokines as well as type I interferons (interferon type I) to help fight viral infection.

The adapter proteins and kinases that mediate TLR signaling have also been targeted. In addition, random germline mutagenesis with ENU has been used to decipher the TLR signaling pathways. When activated, TLRs recruit adapter molecules within the cytoplasm of cells to propagate a signal. Four adapter molecules are known to be involved in signaling. These proteins are known as MyD88, TIRAP (also called Mal), TRIF, and TRAM (TRIF-related adaptor molecule).[51][52][53]

TLR signaling is divided into two distinct signaling pathways, the MyD88-dependent and TRIF-dependent pathway.

MyD88-dependent pathway

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The MyD88-dependent response occurs on dimerization of TLRs, and is used by every TLR except TLR3. Its primary effect is activation of NFκB and Mitogen-activated protein kinase. Ligand binding and conformational change that occurs in the receptor recruits the adaptor protein MyD88, a member of the TIR family. MyD88 then recruits IRAK4, IRAK1 and IRAK2. IRAK kinases then phosphorylate and activate the protein TRAF6, which in turn polyubiquinates the protein TAK1, as well as itself to facilitate binding to IKK-β. On binding, TAK1 phosphorylates IKK-β, which then phosphorylates IκB causing its degradation and allowing NFκB to diffuse into the cell nucleus and activate transcription and consequent induction of inflammatory cytokines.[50]

TRIF-dependent pathway

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Both TLR3 and TLR4 use the TRIF-dependent pathway, which is triggered by dsRNA and LPS, respectively. For TLR3, dsRNA leads to activation of the receptor, recruiting the adaptor TRIF. TRIF activates the kinases TBK1 and RIPK1, which creates a branch in the signaling pathway. The TRIF/TBK1 signaling complex phosphorylates IRF3 allowing its translocation into the nucleus and production of Interferon type I. Meanwhile, activation of RIPK1 causes the polyubiquitination and activation of TAK1 and NFκB transcription in the same manner as the MyD88-dependent pathway.[50]

TLR signaling ultimately leads to the induction or suppression of genes that orchestrate the inflammatory response. In all, thousands of genes are activated by TLR signaling, and collectively, the TLRs constitute one of the most pleiotropic yet tightly regulated gateways for gene modulation.

TLR4 is the only TLR that uses all four adaptors. Complex consisting of TLR4, MD2 and LPS recruits TIR domain-containing adaptors TIRAP and MyD88 and thus initiates activation of NFκB (early phase) and MAPK. TLR4-MD2-LPS complex then undergoes endocytosis and in endosome it forms a signalling complex with TRAM and TRIF adaptors. This TRIF-dependent pathway again leads to IRF3 activation and production of type I interferons, but it also activates late-phase NFκB activation. Both late and early phase activation of NFκB is required for production of inflammatory cytokines.[50]

Medical relevance

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Imiquimod (cardinally used in dermatology) is a TLR7 agonist, and its successor resiquimod, is a TLR7 and TLR8 agonist.[54] Recently, resiquimod has been explored as an agent for cancer immunotherapy,[55] acting through stimulation of tumor-associated macrophages.

Several TLR ligands are in clinical development or being tested in animal models as vaccine adjuvants,[56] with the first clinical use in humans in a recombinant herpes zoster vaccine in 2017, which contains a monophosphoryl lipid A component.

TLR7 messenger RNA expression levels in dairy animals in a natural outbreak of foot-and-mouth disease have been reported.[57]

TLR4 has been shown to be important for the long-term side-effects of opioids. Its activation leads to downstream release of inflammatory modulators including TNF-α and IL-1β, and constant low-level release of these modulators is thought to reduce the efficacy of opioid drug treatment with time, and is involved in opioid tolerance,[58][59] hyperalgesia and allodynia.[60][61] Morphine induced TLR4 activation attenuates pain suppression by opioids and enhances the development of opioid tolerance and addiction, drug abuse, and other negative side effects such as respiratory depression and hyperalgesia.[62] Drugs that block the action of TNF-α or IL-1β have been shown to increase the analgesic effects of opioids and reduce the development of tolerance and other side-effects,[63][64] and this has also been demonstrated with drugs that block TLR4 itself.

The "unnatural" enantiomers of opioid drugs such as (+)-morphine and (+)-naloxone lack affinity for opioid receptors, still produce the same activity at TLR4 as their "normal" enantiomers.[65][66] So, "unnatural" entianomers of opioids such as (+)-naloxone, can be used to block the TLR4 activity of opioid analgesic drugs without having any affinity for μ-opioid receptor[67][66][68]

Discovery

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When microbes were first recognized as the cause of infectious diseases, it was immediately clear that multicellular organisms must be capable of recognizing them when infected and, hence, capable of recognizing molecules unique to microbes. A large body of literature, spanning most of the last century, attests to the search for the key molecules and their receptors. More than 100 years ago, Richard Pfeiffer, a student of Robert Koch, coined the term "endotoxin" to describe a substance produced by Gram-negative bacteria that could provoke fever and shock in experimental animals. In the decades that followed, endotoxin was chemically characterized and identified as a lipopolysaccharide (LPS) produced by most Gram-negative bacteria. This lipopolysaccharide is an integral part of the gram-negative membrane and is released upon destruction of the bacterium. Other molecules (bacterial lipopeptides, flagellin, and unmethylated DNA) were shown in turn to provoke host responses that are normally protective. However, these responses can be detrimental if they are excessively prolonged or intense. It followed logically that there must be receptors for such molecules, capable of alerting the host to the presence of infection, but these remained elusive for many years. Toll-like receptors are now counted among the key molecules that alert the immune system to the presence of microbial infections.

The prototypic member of the family, the toll receptor (P08953; Tl) in the fruit fly Drosophila melanogaster, was discovered in 1985 by 1995 Nobel Laureates Christiane Nüsslein-Volhard and Eric Wieschaus and colleagues. It was known for its developmental function in embryogenesis by establishing the dorsal-ventral axis. It was named after Christiane Nüsslein-Volhard's 1985 exclamation, "Das ist ja toll!" ("That's amazing!"), in reference to the underdeveloped ventral portion of a fruit fly larva.[4] It was cloned by the laboratory of Kathryn Anderson in 1988.[69] In 1996, toll was found by Jules A. Hoffmann and his colleagues to have an essential role in the fly's immunity to fungal infection, which it achieved by activating the synthesis of antimicrobial peptides.[19]

The first reported human toll-like receptor was described by Nomura and colleagues in 1994,[70] mapped to a chromosome by Taguchi and colleagues in 1996.[71] Because the immune function of toll in Drosophila was not then known, it was assumed that TIL (now known as TLR1) might participate in mammalian development. However, in 1991 (prior to the discovery of TIL) it was observed that a molecule with a clear role in immune function in mammals, the interleukin-1 (IL-1) receptor, also had homology to drosophila toll; the cytoplasmic portions of both molecules were similar.[72]

In 1997, Charles Janeway and Ruslan Medzhitov showed that a toll-like receptor now known as TLR4 could, when artificially ligated using antibodies, induce the activation of certain genes necessary for initiating an adaptive immune response.[7] TLR 4 function as an LPS sensing receptor was discovered by Bruce A. Beutler and colleagues.[73] These workers used positional cloning to prove that mice that could not respond to LPS had mutations that abolished the function of TLR4. This identified TLR4 as one of the key components of the receptor for LPS.

The history of Toll-like receptors

In turn, the other TLR genes were ablated in mice by gene targeting, largely in the laboratory of Shizuo Akira and colleagues. Each TLR is now believed to detect a discrete collection of molecules — some of microbial origin, and some products of cell damage — and to signal the presence of infections.[74]

Plant homologs of toll were discovered by Pamela Ronald in 1995 (rice XA21)[75] and Thomas Boller in 2000 (Arabidopsis FLS2).[76]

In 2011, Beutler and Hoffmann were awarded the Nobel Prize in Medicine or Physiology for their work.[77] Hoffmann and Akira received the Canada Gairdner International Award in 2011.[78]

Notes and references

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  1. ^ Mahla RS, Reddy MC, Prasad DV, Kumar H (September 2013). "Sweeten PAMPs: Role of Sugar Complexed PAMPs in Innate Immunity and Vaccine Biology". Frontiers in Immunology. 4: 248. doi:10.3389/fimmu.2013.00248. PMC 3759294. PMID 24032031.
  2. ^ Fore, Faith; Indriputri, Cut; Mamutse, Janet; Nugraha, Jusak (2020). "TLR10 and Its Unique Anti-Inflammatory Properties and Potential Use as a Target in Therapeutics". Immune Network. 20 (3): e21. doi:10.4110/in.2020.20.e21. ISSN 1598-2629. PMC 7327153. PMID 32655969.
  3. ^ Kemball CC, Alirezaei M, Whitton JL (2010). "Type B coxsackieviruses and their interactions with the innate and adaptive immune systems". Future Microbiology. 5 (9): 1329–47. doi:10.2217/fmb.10.101. PMC 3045535. PMID 20860480.
  4. ^ a b Hansson GK, Edfeldt K (June 2005). "Toll to be paid at the gateway to the vessel wall". Arteriosclerosis, Thrombosis, and Vascular Biology. 25 (6): 1085–7. doi:10.1161/01.ATV.0000168894.43759.47. PMID 15923538.
  5. ^ Delneste Y, Beauvillain C, Jeannin P (January 2007). "[Innate immunity: structure and function of TLRs]". Médecine/Sciences. 23 (1): 67–73. doi:10.1051/medsci/200723167. PMID 17212934.
  6. ^ Takeda K, Akira S (January 2005). "Toll-like receptors in innate immunity". International Immunology. 17 (1): 1–14. doi:10.1093/intimm/dxh186. PMID 15585605.
  7. ^ a b c Medzhitov R, Preston-Hurlburt P, Janeway CA (July 1997). "A human homologue of the Drosophila Toll protein signals activation of adaptive immunity". Nature. 388 (6640): 394–7. Bibcode:1997Natur.388..394M. doi:10.1038/41131. PMID 9237759.
  8. ^ a b Sharma N, Akhade AS, Qadri A (April 2013). "Sphingosine-1-phosphate suppresses TLR-induced CXCL8 secretion from human T cells". Journal of Leukocyte Biology. 93 (4): 521–8. doi:10.1189/jlb.0712328. PMID 23345392.
  9. ^ Sameer AS, Nissar S (2021). "Toll-Like Receptors (TLRs): Structure, Functions, Signaling, and Role of Their Polymorphisms in Colorectal Cancer Susceptibility". Biomed Res Int. 2021: 1157023. doi:10.1155/2021/1157023. PMC 8452412. PMID 34552981.
  10. ^ Rolls A, Shechter R, London A, Ziv Y, Ronen A, Levy R, Schwartz M (September 2007). "Toll-like receptors modulate adult hippocampal neurogenesis". Nature Cell Biology. 9 (9): 1081–8. doi:10.1038/ncb1629. PMID 17704767. S2CID 12517461.
  11. ^ Du X, Poltorak A, Wei Y, Beutler B (September 2000). "Three novel mammalian toll-like receptors: gene structure, expression, and evolution". European Cytokine Network. 11 (3): 362–71. PMID 11022119.
  12. ^ Chuang TH, Ulevitch RJ (September 2000). "Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9". European Cytokine Network. 11 (3): 372–8. PMID 11022120.
  13. ^ Tabeta K, Georgel P, Janssen E, Du X, Hoebe K, Crozat K, et al. (March 2004). "Toll-like receptors 9 and 3 as essential components of innate immune defense against mouse cytomegalovirus infection". Proceedings of the National Academy of Sciences of the United States of America. 101 (10): 3516–21. Bibcode:2004PNAS..101.3516T. doi:10.1073/pnas.0400525101. PMC 373494. PMID 14993594.
  14. ^ a b Roach JC, Glusman G, Rowen L, Kaur A, Purcell MK, Smith KD, et al. (July 2005). "The evolution of vertebrate Toll-like receptors". Proceedings of the National Academy of Sciences of the United States of America. 102 (27): 9577–82. Bibcode:2005PNAS..102.9577R. doi:10.1073/pnas.0502272102. PMC 1172252. PMID 15976025.
  15. ^ Lemaitre B, Hoffmann J (2007). "The host defense of Drosophila melanogaster". Annual Review of Immunology. 25: 697–743. doi:10.1146/annurev.immunol.25.022106.141615. PMID 17201680.
  16. ^ Valanne S, Wang JH, Rämet M (January 2011). "The Drosophila Toll signaling pathway". Journal of Immunology. 186 (2): 649–56. doi:10.4049/jimmunol.1002302. PMID 21209287.
  17. ^ a b Dudzic JP, Hanson MA, Iatsenko I, Kondo S, Lemaitre B (April 2019). "More Than Black or White: Melanization and Toll Share Regulatory Serine Proteases in Drosophila". Cell Reports. 27 (4): 1050–61. doi:10.1016/j.celrep.2019.03.101. PMID 31018123.
  18. ^ Hanson MA, Hamilton PT, Perlman SJ (October 2016). "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila". BMC Evolutionary Biology. 16 (1): 228. Bibcode:2016BMCEE..16..228H. doi:10.1186/s12862-016-0805-y. PMC 5078906. PMID 27776480.
  19. ^ a b Lemaitre B, Nicolas E, Michaut L, Reichhart JM, Hoffmann JA (September 1996). "The dorsoventral regulatory gene cassette spätzle/Toll/cactus controls the potent antifungal response in Drosophila adults". Cell. 86 (6): 973–83. doi:10.1016/s0092-8674(00)80172-5. PMID 8808632. S2CID 10736743.
  20. ^ Issa N, Guillaumot N, Lauret E, Matt N, Schaeffer-Reiss C, Van Dorsselaer A, et al. (February 2018). "The Circulating Protease Persephone Is an Immune Sensor for Microbial Proteolytic Activities Upstream of the Drosophila Toll Pathway". Molecular Cell. 69 (4): 539–550.e6. doi:10.1016/j.molcel.2018.01.029. PMC 5823974. PMID 29452635.
  21. ^ Ferrandon D, Imler JL, Hetru C, Hoffmann JA (November 2007). "The Drosophila systemic immune response: sensing and signalling during bacterial and fungal infections". Nature Reviews. Immunology. 7 (11): 862–74. doi:10.1038/nri2194. PMID 17948019. S2CID 11104900.
  22. ^ Chowdhury M, Li CF, He Z, Lu Y, Liu XS, Wang YF, et al. (June 2019). "Drosophila". The Journal of Biological Chemistry. 294 (26): 10172–81. doi:10.1074/jbc.RA118.006804. PMC 6664172. PMID 31088910.
  23. ^ Lee J, Sayed N, Hunter A, Au KF, Wong WH, Mocarski ES, et al. (October 2012). "Activation of innate immunity is required for efficient nuclear reprogramming". Cell. 151 (3): 547–58. doi:10.1016/j.cell.2012.09.034. PMC 3506423. PMID 23101625.
  24. ^ a b Hatai, Hirotsugu; Lepelley, Alice; Zeng, Wangyong; Hayden, Matthew S.; Ghosh, Sankar (2016). "Toll-Like Receptor 11 (TLR11) Interacts with Flagellin and Profilin through Disparate Mechanisms". PLOS ONE. 11 (2): e0148987. Bibcode:2016PLoSO..1148987H. doi:10.1371/journal.pone.0148987. ISSN 1932-6203. PMC 4747465. PMID 26859749.
  25. ^ Mathur R, Oh H, Zhang D, Park SG, Seo J, Koblansky A, et al. (October 2012). "A mouse model of Salmonella typhi infection". Cell. 151 (3): 590–602. doi:10.1016/j.cell.2012.08.042. PMC 3500584. PMID 23101627.
  26. ^ Flajnik, Martin F.; Singh, Nevil J.; Holland, Steven M., eds. (2023). "Ch. 12. Pattern Recognition Receptors and the IL-1 Family". Paul's fundamental immunology (8th ed.). Philadelphia Baltimore New York London Buenos Aires Hong Kong Sydney Tokyo: Wolters Kluwer/Lippincott Williams & Wilkins. ISBN 978-1-9751-4251-3. Broadly, TLRs 1, 2, 4 to 6, and 10 are expressed on the plasma membrane, and TLRs 3, 7 to 9, and 11 to 13 are confined to the endosomes.
  27. ^ a b c Unless else specified in boxes then ref is: Waltenbaugh C, Doan T, Melvold R, Viselli S (2008). Immunology. Lippincott's Illustrated reviews. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins. p. 17. ISBN 978-0-7817-9543-2.
  28. ^ a b Sabroe I, Dower SK, Whyte MK (November 2005). "The role of Toll-like receptors in the regulation of neutrophil migration, activation, and apoptosis". Clinical Infectious Diseases. 41 (Suppl 7): S421-6. doi:10.1086/431992. PMID 16237641.
  29. ^ a b c d Sallusto F, Lanzavecchia A (2002). "The instructive role of dendritic cells on T-cell responses". Arthritis Research. 4 (Suppl 3): S127-32. doi:10.1186/ar567. PMC 3240143. PMID 12110131.
  30. ^ Gerondakis S, Grumont RJ, Banerjee A (2007). "Regulating B-cell activation and survival in response to TLR signals". Immunology and Cell Biology. 85 (6): 471–5. doi:10.1038/sj.icb.7100097. PMID 17637697. S2CID 30443009.
  31. ^ Cario E, Rosenberg IM, Brandwein SL, Beck PL, Reinecker HC, Podolsky DK (January 2000). "Lipopolysaccharide activates distinct signaling pathways in intestinal epithelial cell lines expressing Toll-like receptors". Journal of Immunology. 164 (2): 966–72. doi:10.4049/jimmunol.164.2.966. PMID 10623846.
  32. ^ Peana M, Zdyb K, Medici S, Pelucelli A, Simula G, Gumienna-Kontecka E, Zoroddu MA (December 2017). "Ni(II) interaction with a peptide model of the human TLR4 ectodomain". Journal of Trace Elements in Medicine and Biology. 44: 151–160. Bibcode:2017JTEMB..44..151P. doi:10.1016/j.jtemb.2017.07.006. PMID 28965571.
  33. ^ Salazar Gonzalez RM, Shehata H, O'Connell MJ, Yang Y, Moreno-Fernandez ME, Chougnet CA, Aliberti J (2014). "Toxoplasma gondii- derived profilin triggers human toll-like receptor 5-dependent cytokine production". Journal of Innate Immunity. 6 (5): 685–94. doi:10.1159/000362367. PMC 4141014. PMID 24861338.
  34. ^ Seizer L, Rahimi S, Santos-Sierra S, Drexel M (2022). "Expression of toll like receptor 8 (TLR8) in specific groups of mouse hippocampal interneurons". PLOS ONE. 17 (5): e0267860. Bibcode:2022PLoSO..1767860S. doi:10.1371/journal.pone.0267860. PMC 9067651. PMID 35507634.
  35. ^ Guan Y, Ranoa DR, Jiang S, Mutha SK, Li X, Baudry J, Tapping RI (May 2010). "Human TLRs 10 and 1 share common mechanisms of innate immune sensing but not signaling". Journal of Immunology. 184 (9): 5094–103. doi:10.4049/jimmunol.0901888. PMID 20348427.
  36. ^ Chuang T, Ulevitch RJ (March 2001). "Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1518 (1–2): 157–61. doi:10.1016/s0167-4781(00)00289-x. PMID 11267672.
  37. ^ Hornung V, Rothenfusser S, Britsch S, Krug A, Jahrsdörfer B, Giese T, Endres S, Hartmann G (May 2002). "Quantitative expression of toll-like receptor 1-10 mRNA in cellular subsets of human peripheral blood mononuclear cells and sensitivity to CpG oligodeoxynucleotides". Journal of Immunology. 168 (9): 4531–7. doi:10.4049/jimmunol.168.9.4531. PMID 11970999.
  38. ^ a b Regan T, Nally K, Carmody R, Houston A, Shanahan F, Macsharry J, Brint E (December 2013). "Identification of TLR10 as a key mediator of the inflammatory response to Listeria monocytogenes in intestinal epithelial cells and macrophages". Journal of Immunology. 191 (12): 6084–92. doi:10.4049/jimmunol.1203245. PMID 24198280.
  39. ^ Yarovinsky F, Zhang D, Andersen JF, Bannenberg GL, Serhan CN, Hayden MS, et al. (June 2005). "TLR11 activation of dendritic cells by a protozoan profilin-like protein". Science. 308 (5728): 1626–9. Bibcode:2005Sci...308.1626Y. doi:10.1126/science.1109893. PMID 15860593. S2CID 34165967.
  40. ^ Pifer R, Benson A, Sturge CR, Yarovinsky F (February 2011). "UNC93B1 is essential for TLR11 activation and IL-12-dependent host resistance to Toxoplasma gondii". The Journal of Biological Chemistry. 286 (5): 3307–14. doi:10.1074/jbc.M110.171025. PMC 3030336. PMID 21097503.
  41. ^ Koblansky AA, Jankovic D, Oh H, Hieny S, Sungnak W, Mathur R, et al. (January 2013). "Recognition of profilin by Toll-like receptor 12 is critical for host resistance to Toxoplasma gondii". Immunity. 38 (1): 119–30. doi:10.1016/j.immuni.2012.09.016. PMC 3601573. PMID 23246311.
  42. ^ Mishra BB, Gundra UM, Teale JM (December 2008). "Expression and distribution of Toll-like receptors 11-13 in the brain during murine neurocysticercosis". Journal of Neuroinflammation. 5: 53. doi:10.1186/1742-2094-5-53. PMC 2631477. PMID 19077284.
  43. ^ Shi Z, Cai Z, Sanchez A, Zhang T, Wen S, Wang J, et al. (February 2011). "A novel Toll-like receptor that recognizes vesicular stomatitis virus". The Journal of Biological Chemistry. 286 (6): 4517–24. doi:10.1074/jbc.M110.159590. PMC 3039399. PMID 21131352.
  44. ^ Oldenburg M, Krüger A, Ferstl R, Kaufmann A, Nees G, Sigmund A, et al. (August 2012). "TLR13 recognizes bacterial 23S rRNA devoid of erythromycin resistance-forming modification". Science. 337 (6098): 1111–5. Bibcode:2012Sci...337.1111O. doi:10.1126/science.1220363. PMID 22821982. S2CID 206540638.
  45. ^ Hochrein H, Kirschning CJ (March 2013). "Bacteria evade immune recognition via TLR13 and binding of their 23S rRNA by MLS antibiotics by the same mechanisms". Oncoimmunology. 2 (3): e23141. doi:10.4161/onci.23141. PMC 3661153. PMID 23802068.
  46. ^ Schreibelt, Gerty; Benitez-Ribas, Daniel; Schuurhuis, Danita; Lambeck, Annechien J. A.; van Hout-Kuijer, Maaike; Schaft, Niels; Punt, Cornelis J. A.; Figdor, Carl G.; Adema, Gosse J.; de Vries, I. Jolanda M. (29 July 2010). "Commonly used prophylactic vaccines as an alternative for synthetically produced TLR ligands to mature monocyte-derived dendritic cells". Blood. 116 (4): 564–574. doi:10.1182/blood-2009-11-251884. hdl:2066/89493. ISSN 1528-0020. PMID 20424184.
  47. ^ Aleynick, Mark; Svensson-Arvelund, Judit; Pantsulaia, Gvantsa; Kim, Kristy; Rose, Samuel A.; Upadhyay, Ranjan; Yellin, Michael; Marsh, Henry; Oreper, Daniel; Jhunjhunwala, Suchit; Moussion, Christine Carine; Merad, Miriam; Brown, Brian D.; Brody, Joshua D. (July 2023). "Pattern recognition receptor agonists in pathogen vaccines mediate antitumor T-cell cross-priming". Journal for Immunotherapy of Cancer. 11 (7): e007198. doi:10.1136/jitc-2023-007198. ISSN 2051-1426. PMC 10373699. PMID 37487664.
  48. ^ Hoebe K, Du X, Georgel P, Janssen E, Tabeta K, Kim SO, et al. (August 2003). "Identification of Lps2 as a key transducer of MyD88-independent TIR signalling". Nature. 424 (6950): 743–8. Bibcode:2003Natur.424..743H. doi:10.1038/nature01889. PMID 12872135. S2CID 15608748.
  49. ^ Hemmi H, Takeuchi O, Kawai T, Kaisho T, Sato S, Sanjo H, et al. (December 2000). "A Toll-like receptor recognizes bacterial DNA". Nature. 408 (6813): 740–5. Bibcode:2000Natur.408..740H. doi:10.1038/35047123. PMID 11130078. S2CID 4405163.
  50. ^ a b c d Kawai T, Akira S (May 2010). "The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors". Nature Immunology. 11 (5): 373–84. doi:10.1038/ni.1863. PMID 20404851. S2CID 39414949.
  51. ^ Shigeoka AA, Holscher TD, King AJ, Hall FW, Kiosses WB, Tobias PS, et al. (May 2007). "TLR2 is constitutively expressed within the kidney and participates in ischemic renal injury through both MyD88-dependent and -independent pathways". Journal of Immunology. 178 (10): 6252–8. doi:10.4049/jimmunol.178.10.6252. PMID 17475853.
  52. ^ Yamamoto M, Sato S, Hemmi H, Uematsu S, Hoshino K, Kaisho T, et al. (November 2003). "TRAM is specifically involved in the Toll-like receptor 4-mediated MyD88-independent signaling pathway". Nature Immunology. 4 (11): 1144–50. doi:10.1038/ni986. PMID 14556004. S2CID 13016860.
  53. ^ Yamamoto M, Sato S, Hemmi H, Sanjo H, Uematsu S, Kaisho T, et al. (November 2002). "Essential role for TIRAP in activation of the signalling cascade shared by TLR2 and TLR4". Nature. 420 (6913): 324–9. Bibcode:2002Natur.420..324Y. doi:10.1038/nature01182. PMID 12447441. S2CID 16163262.
  54. ^ Peter Fritsch (2004). Dermatologie Venerologie : Grundlagen. Klinik. Atlas (in German). Berlin: Springer. ISBN 3-540-00332-0.
  55. ^ Rodell CB, Arlauckas SP, Cuccarese MF, Garris CS, Li R, Ahmed MS, et al. (August 2018). "TLR7/8-agonist-loaded nanoparticles promote the polarization of tumour-associated macrophages to enhance cancer immunotherapy". Nature Biomedical Engineering. 2 (8): 578–588. doi:10.1038/s41551-018-0236-8. PMC 6192054. PMID 31015631.
  56. ^ Toussi DN, Massari P (April 2014). "Immune Adjuvant Effect of Molecularly-defined Toll-Like Receptor Ligands". Vaccines. 2 (2): 323–53. doi:10.3390/vaccines2020323. PMC 4494261. PMID 26344622.
  57. ^ Audarya, S.D.; Pattnaik, B.; Sanyal, A.; Mohapatra, J.K. (2017). "Toll like Receptor 7 Messenger Ribonucleic Acid Expression Levels in Dairy Animals in an Outbreak of Foot-and-mouth disease" (PDF). Buffalo Bulletin. 36 (3): 489–495. Archived from the original (PDF) on 28 April 2021.
  58. ^ Shavit Y, Wolf G, Goshen I, Livshits D, Yirmiya R (May 2005). "Interleukin-1 antagonizes morphine analgesia and underlies morphine tolerance". Pain. 115 (1–2): 50–9. doi:10.1016/j.pain.2005.02.003. PMID 15836969. S2CID 7286123.
  59. ^ Mohan S, Davis RL, DeSilva U, Stevens CW (October 2010). "Dual regulation of mu opioid receptors in SK-N-SH neuroblastoma cells by morphine and interleukin-1β: evidence for opioid-immune crosstalk". Journal of Neuroimmunology. 227 (1–2): 26–34. doi:10.1016/j.jneuroim.2010.06.007. PMC 2942958. PMID 20615556.
  60. ^ Komatsu T, Sakurada S, Katsuyama S, Sanai K, Sakurada T (2009). Mechanism of allodynia evoked by intrathecal morphine-3-glucuronide in mice. International Review of Neurobiology. Vol. 85. pp. 207–19. doi:10.1016/S0074-7742(09)85016-2. ISBN 978-0-12-374893-5. PMID 19607972.
  61. ^ Lewis SS, Hutchinson MR, Rezvani N, Loram LC, Zhang Y, Maier SF, Rice KC, Watkins LR (January 2010). "Evidence that intrathecal morphine-3-glucuronide may cause pain enhancement via toll-like receptor 4/MD-2 and interleukin-1beta". Neuroscience. 165 (2): 569–83. doi:10.1016/j.neuroscience.2009.10.011. PMC 2795035. PMID 19833175.
  62. ^ Drahl C (22 August 2012). "Small Molecules Target Toll-Like Receptors". Chemical & Engineering News.
  63. ^ Shen CH, Tsai RY, Shih MS, Lin SL, Tai YH, Chien CC, Wong CS (February 2011). "Etanercept restores the antinociceptive effect of morphine and suppresses spinal neuroinflammation in morphine-tolerant rats". Anesthesia and Analgesia. 112 (2): 454–9. doi:10.1213/ANE.0b013e3182025b15. PMID 21081778. S2CID 12295407.
  64. ^ Hook MA, Washburn SN, Moreno G, Woller SA, Puga D, Lee KH, Grau JW (February 2011). "An IL-1 receptor antagonist blocks a morphine-induced attenuation of locomotor recovery after spinal cord injury". Brain, Behavior, and Immunity. 25 (2): 349–59. doi:10.1016/j.bbi.2010.10.018. PMC 3025088. PMID 20974246.
  65. ^ Watkins LR, Hutchinson MR, Rice KC, Maier SF (November 2009). "The "toll" of opioid-induced glial activation: improving the clinical efficacy of opioids by targeting glia". Trends in Pharmacological Sciences. 30 (11): 581–91. doi:10.1016/j.tips.2009.08.002. PMC 2783351. PMID 19762094.
  66. ^ a b Hutchinson MR, Zhang Y, Brown K, Coats BD, Shridhar M, Sholar PW, Patel SJ, Crysdale NY, Harrison JA, Maier SF, Rice KC, Watkins LR (July 2008). "Non-stereoselective reversal of neuropathic pain by naloxone and naltrexone: involvement of toll-like receptor 4 (TLR4)". The European Journal of Neuroscience. 28 (1): 20–9. doi:10.1111/j.1460-9568.2008.06321.x. PMC 2588470. PMID 18662331.
  67. ^ Hutchinson MR, Coats BD, Lewis SS, Zhang Y, Sprunger DB, Rezvani N, Baker EM, Jekich BM, Wieseler JL, Somogyi AA, Martin D, Poole S, Judd CM, Maier SF, Watkins LR (November 2008). "Proinflammatory cytokines oppose opioid-induced acute and chronic analgesia". Brain, Behavior, and Immunity. 22 (8): 1178–89. doi:10.1016/j.bbi.2008.05.004. PMC 2783238. PMID 18599265.
  68. ^ Hutchinson MR, Lewis SS, Coats BD, Rezvani N, Zhang Y, Wieseler JL, Somogyi AA, Yin H, Maier SF, Rice KC, Watkins LR (May 2010). "Possible involvement of toll-like receptor 4/myeloid differentiation factor-2 activity of opioid inactive isomers causes spinal proinflammation and related behavioral consequences". Neuroscience. 167 (3): 880–93. doi:10.1016/j.neuroscience.2010.02.011. PMC 2854318. PMID 20178837.
  69. ^ Hashimoto C, Hudson KL, Anderson KV (January 1988). "The Toll gene of Drosophila, required for dorsal-ventral embryonic polarity, appears to encode a transmembrane protein". Cell. 52 (2): 269–79. doi:10.1016/0092-8674(88)90516-8. PMID 2449285. S2CID 19439405.
  70. ^ Nomura N, Miyajima N, Sazuka T, Tanaka A, Kawarabayasi Y, Sato S, et al. (1994). "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1". DNA Research. 1 (1): 27–35. doi:10.1093/dnares/1.1.27. PMID 7584026.
  71. ^ Taguchi T, Mitcham JL, Dower SK, Sims JE, Testa JR (March 1996). "Chromosomal localization of TIL, a gene encoding a protein related to the Drosophila transmembrane receptor Toll, to human chromosome 4p14". Genomics. 32 (3): 486–8. doi:10.1006/geno.1996.0150. PMID 8838819.
  72. ^ Gay NJ, Keith FJ (May 1991). "Drosophila Toll and IL-1 receptor". Nature. 351 (6325): 355–6. Bibcode:1991Natur.351..355G. doi:10.1038/351355b0. PMID 1851964. S2CID 1700458.
  73. ^ Poltorak A, He X, Smirnova I, Liu MY, Van Huffel C, Du X, et al. (December 1998). "Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene". Science. 282 (5396): 2085–8. Bibcode:1998Sci...282.2085P. doi:10.1126/science.282.5396.2085. PMID 9851930.
  74. ^ Hoshino K, Takeuchi O, Kawai T, Sanjo H, Ogawa T, Takeda Y, et al. (April 1999). "Cutting edge: Toll-like receptor 4 (TLR4)-deficient mice are hyporesponsive to lipopolysaccharide: evidence for TLR4 as the Lps gene product". Journal of Immunology. 162 (7): 3749–52. doi:10.4049/jimmunol.162.7.3749. PMID 10201887. S2CID 7419784.
  75. ^ Song WY, Wang GL, Chen LL, Kim HS, Pi LY, Holsten T, et al. (December 1995). "A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21". Science. 270 (5243): 1804–6. Bibcode:1995Sci...270.1804S. doi:10.1126/science.270.5243.1804. PMID 8525370. S2CID 10548988.
  76. ^ Gómez-Gómez L, Boller T (June 2000). "FLS2: an LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis". Molecular Cell. 5 (6): 1003–11. doi:10.1016/S1097-2765(00)80265-8. PMID 10911994.
  77. ^ "The Nobel Prize in Physiology or Medicine 2011". Nobel Media AB. 3 October 2011.
  78. ^ Mitchell B (23 March 2011). "B.C. doctor wins prestigious medical prize". The Star.

See also

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